中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (34): 5545-5551.doi: 10.3969/j.issn.2095-4344.2017.34.022

• 生物材料综述 biomaterial review • 上一篇    下一篇

脱细胞肝脏支架制备及再细胞化:生物人工肝离我们还有多远?

苏文君,张  蕾
  

  1. 昆明医科大学附属甘美医院/昆明市第一人民医院,云南省昆明市  650011
  • 收稿日期:2017-07-11 出版日期:2017-12-08 发布日期:2018-01-04
  • 通讯作者: 张蕾,博士,研究员,昆明医科大学附属甘美医院/昆明市第一人民医院,云南省昆明市 650011
  • 作者简介:苏文君,女,1988年生,云南省昆明市人,汉族,2013年云南大学生命科学学院毕业,硕士,研究实习员,主要从事组织工程方面的研究工作。
  • 基金资助:

    国家自然科学基金项目(81660303)

Decellularized liver scaffold’s preparation and recellularization: how far is the bioartificial liver from clinical practice?  

Su Wen-jun, Zhang Lei
  

  1. Affiliated Calmette Hospital of Kunming Medical University/First Hospital of Kunming, Kunming 650011, Yunnan Province, China
  • Received:2017-07-11 Online:2017-12-08 Published:2018-01-04
  • Contact: Zhang Lei, Ph.D., Senior researcher, Affiliated Calmette Hospital of Kunming Medical University/First Hospital of Kunming, Kunming 650011, Yunnan Province, China
  • About author:Su Wen-jun, Master, Research intern, Biomedical Research Center, Affiliated Calmette Hospital of Kunming Medical University/First Hospital of Kunming, Kunming 650011, Yunnan Province, China
  • Supported by:
    the National Natural Science Foundation of China in 2017, No. 81660303

摘要:

文章快速阅读:

 

文题释义:
肝脏脱细胞支架:通过化学方法、物理方法或酶解方法来破坏肝脏组织中的细胞结构,然后从肝脏组织中去除细胞,尽量保留细胞外基质及肝组织的三维结构,形成无免疫原性或低免疫原性的肝脏组织工程脱细胞支架,近年来逐渐成为组织工程研究的热点。
再细胞化技术:将一定数量的种子细胞一次性或分步移植入3D脱细胞支架内,种子细胞在支架内存活、黏附、增殖、迁移,并发挥肝细胞特定功能,是制备生物人工肝的关键步骤。
 
背景:临床上已展开了大量生物人工肝脏的相关研究,其中肝脏脱细胞支架是已知最有前景的人工肝脏支架材料。
目的:对现阶段3D肝脏脱细胞支架的制备、鉴定、应用等研究现状进行总结,并展望其未来在临床上的发展方向。
方法:检索 PubMed 数据库、中国知网数据库(CNKI)2001年1月至2016年11月期间有关3D肝脏脱细胞支架的相关文献。中文检索词为脱细胞支架,种子细胞,再细胞化,动物实验;英文检索词为decellularized liver scaffolds,recellularization,bioengineered liver。共64篇文献纳入分析并进行引用及讨论。
结果与结论:采用化学方法、物理方法或酶消化法将细胞从肝脏组织中移除,保留完整的细胞外基质三维结构,然后对该支架进行再细胞化,可制得生物人工肝。研究显示将生物人工肝移植入动物体内,可短时间代偿肝脏功能,在未来,将这类生物人工肝真正应用于临床仍需要面临巨大挑战。

关键词: 生物材料, 肝脏脱细胞支架, 种子细胞, 再细胞化, 生物人工肝脏, 动物实验, 国家自然科学基金

Abstract:

BACKGROUND: A lot of clinical studies on bioartificial liver have been launched. Decellularized liver scaffolds are known as the most promising scaffold material of artificial liver.
OBJECTIVE: To summarize the research status of preparation, evaluation and application of three-dimensional (3D) decellularized liver scaffolds, and to discuss the future development in clinical practice.
METHODS: The articles about 3D decellularized liver scaffolds were retrieved from PubMed and China National  Knowledge Infrastructure databases with the key words of “decellularized liver scaffolds, recellularization, bioengineered liver”, and the retrieval period was from January 2001 to November 2016. Repetitive articles were excluded, and 64 articles were included for final review.
RESULTS AND CONCLUSION: To prepare 3D decellularized liver scaffolds, the cells were removed from the liver tissues by chemical or physical methods for which the 3D structure of the extracellular matrix could be preserved intact. After a series of reseeding process, we could finally produce the bioartificial liver. Research has shown that when the bioartificial liver is transplanted into animals, it will compensate the function of liver within a short time. However, it still faces enormous challenges to see whether the bioartificial liver could be used in the clinic. 

Key words: Tissue Scaffolds, Liver, Artificial, Tissue Engineering

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